2 March 2017 Motion characterization scheme to minimize motion artifacts in intravital microscopy
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Abstract
Respiratory- and cardiac-induced motion artifacts pose a major challenge for in vivo optical imaging, limiting the temporal and spatial imaging resolution in fluorescence laser scanning microscopy. Here, we present an imaging platform developed for in vivo characterization of physiologically induced axial motion. The motion characterization system can be straightforwardly implemented on any conventional laser scanning microscope and can be used to evaluate the effectiveness of different motion stabilization schemes. This method is particularly useful to improve the design of novel tissue stabilizers and to facilitate stabilizer positioning in real time, therefore facilitating optimal tissue immobilization and minimizing motion induced artifacts.
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)
Sungon Lee, Sungon Lee, Gabriel Courties, Gabriel Courties, Matthias Nahrendorf, Matthias Nahrendorf, Ralph Weissleder, Ralph Weissleder, Claudio Vinegoni, Claudio Vinegoni, } "Motion characterization scheme to minimize motion artifacts in intravital microscopy," Journal of Biomedical Optics 22(3), 036005 (2 March 2017). https://doi.org/10.1117/1.JBO.22.3.036005 . Submission: Received: 1 July 2016; Accepted: 13 February 2017
Received: 1 July 2016; Accepted: 13 February 2017; Published: 2 March 2017
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