Ultraminiature optical design for multispectral fluorescence imaging endoscopes

Tyler H. Tate; Molly Keenan; John F. Black; Urs Utzinger; Jennifer K. Barton

doi:10.1117/1.JBO.22.3.036013

23 March 2017 Ultraminiature optical design for multispectral fluorescence imaging endoscopes

Tyler H. Tate; Molly Keenan; John F. Black; Urs Utzinger; Jennifer K. Barton

Author Affiliations +

J. of Biomedical Optics, 22(3), 036013 (2017). https://doi.org/10.1117/1.JBO.22.3.036013

Abstract

A miniature wide-field multispectral endoscopic imaging system was developed enabling reflectance and fluorescence imaging over a broad wavelength range. At 0.8-mm diameter, the endoscope can be utilized for natural orifice imaging in small lumens such as the fallopian tubes. Five lasers from 250 to 642 nm are coupled into a 125 - μ m diameter multimode fiber and transmitted to the endoscope distal tip for illumination. Ultraviolet and blue wavelengths excite endogenous fluorophores, which can provide differential fluorescence emission images for health and disease. Visible wavelengths provide reflectance images that can be combined for pseudo-white-light imaging and navigation. Imaging is performed by a 300 - μ m diameter three-element lens system connected to a 3000-element fiber. The lens system was designed for a 70-deg full field of view, working distance from 3 mm to infinity, and 40% contrast at the Nyquist cutoff of the fiber bundle. Measured performance characteristics are near design goals. The endoscope was utilized to obtain example monochromatic, pseudo-white-light, and composite fluorescence images of phantoms and porcine reproductive tract. This work shows the feasibility of packaging a highly capable multispectral fluorescence imaging system into a miniature endoscopic system that may have applications in early detection of cancer.

Citation Download Citation

Tyler H. Tate, Tyler H. Tate, Molly Keenan, Molly Keenan, John F. Black, John F. Black, Urs Utzinger, Urs Utzinger, Jennifer K. Barton, Jennifer K. Barton, } "Ultraminiature optical design for multispectral fluorescence imaging endoscopes," Journal of Biomedical Optics 22(3), 036013 (23 March 2017). https://doi.org/10.1117/1.JBO.22.3.036013 . Submission: Received: 21 October 2016; Accepted: 6 March 2017

Received: 21 October 2016; Accepted: 6 March 2017; Published: 23 March 2017

ACCESS THE FULL ARTICLE

PERSONAL SIGN IN
Full access may be available with your subscription

Email or Username Forgot your username?

Password Forgot your password?

Show

Remember Email/Username on this computer

Remember Password

No SPIE account? Create an account
or Institutional Sign In via Shibboleth

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $20.00

Non-members: $25.00 ADD TO CART

Show All Keywords

Keywords/Phrases

Search In:

Publication Years