Diffuse fluorescence fiber probe for in vivo detection of circulating cells

Vivian E. Pera; Xuefei Tan; Judith M. Runnels; Neha R. Sardesai; Charles P. Lin; Mark Niedre

doi:10.1117/1.JBO.22.3.037004

14 March 2017 Diffuse fluorescence fiber probe for in vivo detection of circulating cells

Vivian E. Pera, Xuefei Tan, Judith M. Runnels, Neha R. Sardesai, Charles P. Lin, Mark Niedre

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Journal of Biomedical Optics, Vol. 22, Issue 3, 037004 (March 2017). https://doi.org/10.1117/1.JBO.22.3.037004

Abstract

There has been significant recent interest in the development of technologies for enumeration of rare circulating cells directly in the bloodstream in many areas of research, for example, in small animal models of circulating tumor cell dissemination during cancer metastasis. We describe a fiber-based optical probe that allows fluorescence detection of labeled circulating cells in vivo in a diffuse reflectance configuration. We validated this probe in a tissue-mimicking flow phantom model in vitro and in nude mice injected with fluorescently labeled multiple myeloma cells in vivo. Compared to our previous work, this design yields an improvement in detection signal-to-noise ratio of 10 dB, virtually eliminates problematic motion artifacts due to mouse breathing, and potentially allows operation in larger animals and limbs.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Vivian E. Pera, Xuefei Tan, Judith M. Runnels, Neha R. Sardesai, Charles P. Lin, and Mark Niedre "Diffuse fluorescence fiber probe for in vivo detection of circulating cells," Journal of Biomedical Optics 22(3), 037004 (14 March 2017). https://doi.org/10.1117/1.JBO.22.3.037004

Received: 5 January 2017; Accepted: 22 February 2017; Published: 14 March 2017

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