30 June 2017 Multiphoton autofluorescence lifetime imaging of induced pluripotent stem cells
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Abstract
The multiphoton fluorescence lifetime imaging tomograph MPTflex with its flexible 360-deg scan head, articulated arm, and tunable femtosecond laser source was employed to study induced pluripotent stem cell (iPS) cultures. Autofluorescence (AF) lifetime imaging was performed with 250-ps temporal resolution and submicron spatial resolution using time-correlated single-photon counting. The two-photon excited AF was based on the metabolic coenzymes NAD(P)H and flavin adenine dinucleotide/flavoproteins. iPS cells generated from mouse embryonic fibroblasts (MEFs) and cocultured with growth-arrested MEFs as feeder cells have been studied. Significant differences on AF lifetime signatures were identified between iPS and feeder cells as well as between their differentiating counterparts.
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)
Aisada Uchugonova, "Multiphoton autofluorescence lifetime imaging of induced pluripotent stem cells," Journal of Biomedical Optics 22(6), 066018 (30 June 2017). https://doi.org/10.1117/1.JBO.22.6.066018 . Submission: Received: 23 February 2017; Accepted: 7 June 2017
Received: 23 February 2017; Accepted: 7 June 2017; Published: 30 June 2017
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