Modulation of ultrasound-switchable fluorescence for improving signal-to-noise ratio

Jayanth Kandukuri; Shuai Yu; Tingfeng Yao; Baohong Yuan

doi:10.1117/1.JBO.22.7.076021

Abstract

Simultaneously achieving high signal-to-noise ratio (SNR) (or sensitivity) and high resolution is desired in biomedical imaging. However, conventional imaging modality has a tradeoff between SNR (or sensitivity) and resolution. We developed a method to simultaneously achieve high SNR (or sensitivity) and high resolution for fluorescence imaging in deep tissue. We first introduce a recently developed deep-tissue high-resolution imaging technique termed as ultrasound-switchable fluorescence (USF). An approach of modulating ultrasound exposure time is adopted to increase the detectability of the USF signal. The control parameters of modulation of ultrasound—such as (1) frequency, (2) duty cycle, and (3) exposure duration—are varied to study their influence on the USF signal and SNR. We conclude that high SNR can be achieved by modulating ultrasound exposure without sacrificing the spatial resolution. This is important for future fluorescence molecular imaging of cancer in deep tissue.

Citation Download Citation

Jayanth Kandukuri, Jayanth Kandukuri, Shuai Yu, Shuai Yu, Tingfeng Yao, Tingfeng Yao, Baohong Yuan, Baohong Yuan, } "Modulation of ultrasound-switchable fluorescence for improving signal-to-noise ratio," Journal of Biomedical Optics 22(7), 076021 (31 July 2017). https://doi.org/10.1117/1.JBO.22.7.076021 . Submission: Received: 8 May 2017; Accepted: 14 July 2017

Received: 8 May 2017; Accepted: 14 July 2017; Published: 31 July 2017

ACCESS THE FULL ARTICLE

PERSONAL SIGN IN
Full access may be available with your subscription

Email or Username Forgot your username?

Password Forgot your password?

Show

Remember Email/Username on this computer

Remember Password

No SPIE account? Create an account
or Institutional Sign In via Shibboleth

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $20.00

Non-members: $25.00 ADD TO CART

Keywords/Phrases

Search In:

Publication Years