31 August 2017 Evaluating biomechanical properties of murine embryos using Brillouin microscopy and optical coherence tomography
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J. of Biomedical Optics, 22(8), 086013 (2017). doi:10.1117/1.JBO.22.8.086013
Abstract
Embryogenesis is regulated by numerous changes in mechanical properties of the cellular microenvironment. Thus, studying embryonic mechanophysiology can provide a more thorough perspective of embryonic development, potentially improving early detection of congenital abnormalities as well as evaluating and developing therapeutic interventions. A number of methods and techniques have been used to study cellular biomechanical properties during embryogenesis. While some of these techniques are invasive or involve the use of external agents, others are compromised in terms of spatial and temporal resolutions. We propose the use of Brillouin microscopy in combination with optical coherence tomography (OCT) to measure stiffness as well as structural changes in a developing embryo. While Brillouin microscopy assesses the changes in stiffness among different organs of the embryo, OCT provides the necessary structural guidance.
Raksha Raghunathan, Jitao Zhang, Chen Wu, Justin Rippy, Manmohan Singh, Kirill V. Larin, Giuliano Scarcelli, "Evaluating biomechanical properties of murine embryos using Brillouin microscopy and optical coherence tomography," Journal of Biomedical Optics 22(8), 086013 (31 August 2017). http://dx.doi.org/10.1117/1.JBO.22.8.086013
Submission: Received 8 June 2017; Accepted 3 August 2017
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KEYWORDS
Optical coherence tomography

Microscopy

Light scattering

Optical microscopy

Tissues

Heart

Temporal resolution

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