23 August 2017 Protease sensing using nontoxic silicon quantum dots
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Abstract
Herein is presented a proof-of-concept study of protease sensing that combines nontoxic silicon quantum dots (SiQDs) with Förster resonance energy transfer (FRET). The SiQDs serve as the donor and an organic dye as the acceptor. The dye is covalently attached to the SiQDs using a peptide linker. Enzymatic cleavage of the peptide leads to changes in FRET efficiency. The combination of interfacial design and optical imaging presented in this work opens opportunities for use of nontoxic SiQDs relevant to intracellular sensing and imaging.
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)
Xiaoyu Cheng, Xiaoyu Cheng, Benjamin F. P. McVey, Benjamin F. P. McVey, Andrew B. Robinson, Andrew B. Robinson, Guillaume Longatte, Guillaume Longatte, Peter B. O’Mara, Peter B. O’Mara, Vincent T. G. Tan, Vincent T. G. Tan, Pall Thordarson, Pall Thordarson, Richard D. Tilley, Richard D. Tilley, Katharina Gaus, Katharina Gaus, J. Justin Gooding, J. Justin Gooding, } "Protease sensing using nontoxic silicon quantum dots," Journal of Biomedical Optics 22(8), 087002 (23 August 2017). https://doi.org/10.1117/1.JBO.22.8.087002 . Submission: Received: 25 April 2017; Accepted: 31 July 2017
Received: 25 April 2017; Accepted: 31 July 2017; Published: 23 August 2017
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