25 March 2017 Low-frequency vibrational spectroscopy of proteins with different secondary structures
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Abstract
Fourier transform infrared (FTIR) and Raman spectra of proteins with significantly different structures are measured in a spectral interval of 50 to 500    cm 1 and noticeable spectral differences are revealed. Intensities of several spectral bands correlate with contents of secondary structure elements. FTIR spectra of superhelical proteins exhibit developed spectral features that are absent in the spectra of globular proteins. Significant differences of the Raman spectra of proteins that are not directly related to the difference of the secondary structures can be due to differences of tertiary and/or quaternary structure of protein molecules.
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)
Irina A. Balakhnina, Irina A. Balakhnina, Nikolay N. Brandt, Nikolay N. Brandt, Andrey Y. Chikishev, Andrey Y. Chikishev, Anna A. Mankova, Anna A. Mankova, Irina G. Shpachenko, Irina G. Shpachenko, } "Low-frequency vibrational spectroscopy of proteins with different secondary structures," Journal of Biomedical Optics 22(9), 091509 (25 March 2017). https://doi.org/10.1117/1.JBO.22.9.091509 . Submission: Received: 5 January 2017; Accepted: 14 March 2017
Received: 5 January 2017; Accepted: 14 March 2017; Published: 25 March 2017
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