18 April 2017 Assessment of decellularization of heart bioimplants using a Raman spectroscopy method
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Abstract
We report the results of experimental studies on cardiac implants using a Raman spectroscopy method (RS). Raman spectra characteristics of leaves and walls of cardiac implants were obtained; the implants were manufactured by protocols of detergent-enzymatic technique (DET) and biological, detergent-free (BIO) decellularization, using detergents (group DET) or a detergent-free, nonproteolytic, actin-disassembling regimen (BIO). There were input optical coefficients that allowed us to carry out evaluation of the protocols of DET and BIO decellularization on the basis of the concentrations of glycosaminoglycans, proteins, amides, and DNA. It was shown that during DET and BIO decellularization, composition aberrations of proteins and lipids do not occur and the integrity of the collagenous structures is preserved. It was found that during the DET decellularization, preservation of glycosaminoglycans is better than during BIO decellularization.
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)
Elena V. Timchenko, Elena V. Timchenko, Pavel E. Timchenko, Pavel E. Timchenko, Artur Lichtenberg, Artur Lichtenberg, Alexander Assmann, Alexander Assmann, Hug Aubin, Hug Aubin, Payam Akhyari, Payam Akhyari, Larisa T. Volova, Larisa T. Volova, Svetlana V. Pershutkina, Svetlana V. Pershutkina, } "Assessment of decellularization of heart bioimplants using a Raman spectroscopy method," Journal of Biomedical Optics 22(9), 091511 (18 April 2017). https://doi.org/10.1117/1.JBO.22.9.091511 . Submission: Received: 3 December 2016; Accepted: 22 March 2017
Received: 3 December 2016; Accepted: 22 March 2017; Published: 18 April 2017
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