25 April 2017 Off-axis holographic laser speckle contrast imaging of blood vessels in tissues
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Abstract
Laser speckle contrast imaging (LSCI) has become one of the most common tools for functional imaging in tissues. Incomplete theoretical description and sophisticated interpretation of measurement results are completely sidelined by a low-cost and simple hardware, fastness, consistent results, and repeatability. In addition to the relatively low measuring volume with around 700    μ m of the probing depth for the visible spectral range of illumination, there is no depth selectivity in conventional LSCI configuration; furthermore, in a case of high NA objective, the actual penetration depth of light in tissues is greater than depth of field (DOF) of an imaging system. Thus, the information about these out-of-focus regions persists in the recorded frames but cannot be retrieved due to intensity-based registration method. We propose a simple modification of LSCI system based on the off-axis holography to introduce after-registration refocusing ability to overcome both depth-selectivity and DOF problems as well as to get the potential possibility of producing a cross-section view of the specimen.
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)
Arkady Abdurashitov, Arkady Abdurashitov, Olga Bragina, Olga Bragina, Olga Sindeeva, Olga Sindeeva, Sergey Sindeev, Sergey Sindeev, Oxana V. Semyachkina-Glushkovskaya, Oxana V. Semyachkina-Glushkovskaya, Valery V. Tuchin, Valery V. Tuchin, } "Off-axis holographic laser speckle contrast imaging of blood vessels in tissues," Journal of Biomedical Optics 22(9), 091514 (25 April 2017). https://doi.org/10.1117/1.JBO.22.9.091514 . Submission: Received: 31 December 2016; Accepted: 27 March 2017
Received: 31 December 2016; Accepted: 27 March 2017; Published: 25 April 2017
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