14 September 2017 FRET-based method for evaluation of the efficiency of reversible and irreversible sonoporation
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Abstract
It is widely known that not all of the treated cells survive after introduction of exogenous molecules via any physical method. Therefore, it is important to develop methods that would allow simultaneous evaluation of both molecular delivery efficiency and cell viability. This study presents Förster resonance energy transfer (FRET)-based method that allows molecular transfer and cell viability evaluation in a single measurement by employing two common fluorescent dyes, namely, ethidium bromide and trypan blue. The method has been validated using cell sonoporation. The FRET-based method allows the efficiency evaluation of both reversible and irreversible sonoporation in a single experiment. Therefore, this method could be used to reduce time, labor, and material cost while improving the accuracy of evaluations.
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)
Paulius Ruzgys, Paulius Ruzgys, Mindaugas Tamošiūnas, Mindaugas Tamošiūnas, Vanesa Lukinsone, Vanesa Lukinsone, Saulius Šatkauskas, Saulius Šatkauskas, } "FRET-based method for evaluation of the efficiency of reversible and irreversible sonoporation," Journal of Biomedical Optics 22(9), 097001 (14 September 2017). https://doi.org/10.1117/1.JBO.22.9.097001 . Submission: Received: 30 March 2017; Accepted: 21 August 2017
Received: 30 March 2017; Accepted: 21 August 2017; Published: 14 September 2017
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