16 January 2018 Improved axial point spread function in a two-frequency laser scanning confocal fluorescence microscope
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Abstract
A two-frequency laser scanning confocal fluorescence microscope (TF-LSCFM) based on intensity modulated fluorescence signal detection was proposed. The specimen-induced spherical aberration and scattering effect were suppressed intrinsically, and high image contrast was presented due to heterodyne interference. An improved axial point spread function in a TF-LSCFM compared with a conventional laser scanning confocal fluorescence microscope was demonstrated and discussed.
© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE)
Jheng-Syong Wu, Jheng-Syong Wu, Yung-Chin Chung, Yung-Chin Chung, Jun-Jei Chien, Jun-Jei Chien, Chien Chou, Chien Chou, } "Improved axial point spread function in a two-frequency laser scanning confocal fluorescence microscope," Journal of Biomedical Optics 23(1), 010502 (16 January 2018). https://doi.org/10.1117/1.JBO.23.1.010502 . Submission: Received: 21 April 2017; Accepted: 13 December 2017
Received: 21 April 2017; Accepted: 13 December 2017; Published: 16 January 2018
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