16 January 2018 Intrinsic fluorescence for cervical precancer detection using polarized light based in-house fabricated portable device
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Abstract
An in-house fabricated portable device has been tested to detect cervical precancer through the intrinsic fluorescence from human cervix of the whole uterus in a clinical setting. A previously validated technique based on simultaneously acquired polarized fluorescence and polarized elastic scattering spectra from a turbid medium is used to extract the intrinsic fluorescence. Using a diode laser at 405 nm, intrinsic fluorescence of flavin adenine dinucleotide, which is the dominant fluorophore and other contributing fluorophores in the epithelium of cervical tissue, has been extracted. Different grades of cervical precancer (cervical intraepithelial neoplasia; CIN) have been discriminated using principal component analysis-based Mahalanobis distance and linear discriminant analysis. Normal, CIN I and CIN II samples have been discriminated from one another with high sensitivity and specificity at 95% confidence level. This ex vivo study with cervix of whole uterus samples immediately after hysterectomy in a clinical environment indicates that the in-house fabricated portable device has the potential to be used as a screening tool for in vivo precancer detection using intrinsic fluorescence.
© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE)
Bharat Lal Meena, Pankaj Singh, Amar Nath Sah, Kiran Pandey, Asha Agarwal, Chayanika Pantola, Asima Pradhan, "Intrinsic fluorescence for cervical precancer detection using polarized light based in-house fabricated portable device," Journal of Biomedical Optics 23(1), 015005 (16 January 2018). https://doi.org/10.1117/1.JBO.23.1.015005 . Submission: Received: 8 August 2017; Accepted: 21 December 2017
Received: 8 August 2017; Accepted: 21 December 2017; Published: 16 January 2018
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