1 October 2018 Structured polarized light microscopy for collagen fiber structure and orientation quantification in thick ocular tissues
Author Affiliations +
Abstract
Collagen is a major constituent of the eye and understanding its architecture and biomechanics is critical to preserve and restore vision. We, recently, demonstrated polarized light microscopy (PLM) as a powerful technique for measuring properties of the collagen fibers of the eye, such as spatial distribution and orientation. Our implementation of PLM, however, required sectioning the tissues for imaging using transmitted light. This is problematic because it limits analysis to thin sections. This is not only slow, but precludes study of dynamic events such as pressure-induced deformations, which are central to the role of collagen. We introduce structured polarized light microscopy (SPLM), an imaging technique that combines structured light illumination with PLM to allow imaging and measurement of collagen fiber properties in thick ocular tissues. Using pig and sheep eyes, we show that SPLM rejects diffuse background light effectively in thick tissues, significantly enhancing visualization of optic nerve head (ONH) structures, such as the lamina cribrosa, and improving the accuracy of the collagen fiber orientation measurements. Further, we demonstrate the integration of SPLM with an inflation device to enable direct visualization, deformation tracking, and quantification of collagen fibers in ONHs while under controlled pressure.
© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE)
Bin Yang, Bin Yang, Bryn Brazile, Bryn Brazile, Ning-Jiun Jan, Ning-Jiun Jan, Yi Hua, Yi Hua, Junchao Wei, Junchao Wei, Ian A. Sigal, Ian A. Sigal, } "Structured polarized light microscopy for collagen fiber structure and orientation quantification in thick ocular tissues," Journal of Biomedical Optics 23(10), 106001 (1 October 2018). https://doi.org/10.1117/1.JBO.23.10.106001 . Submission: Received: 2 July 2018; Accepted: 13 September 2018
Received: 2 July 2018; Accepted: 13 September 2018; Published: 1 October 2018
JOURNAL ARTICLE
10 PAGES


SHARE
Back to Top