6 February 2018 <italic<Limulus</italic< amoebocyte lysate test via an open-microcavity optical biosensor
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J. of Biomedical Optics, 23(2), 027001 (2018). doi:10.1117/1.JBO.23.2.027001
Almost since its discovery, Limulus amoebocyte lysate (LAL) testing has been an important part of the pharmaceutical quality control toolkit. It allows for in vitro endotoxin testing, which has replaced tests using animals, such as using rabbits’ thermal response to judge pyrogenicity of test samples, thus leading to a less expensive and faster test of parenteral pharmaceuticals and medical devices that contact blood or cerebrospinal fluid. However, limited by the detection mechanisms of the LAL assays currently used in industry, further improvement in their performance is challenging. To address the growing demand on optimizing LAL assays for increased test sensitivity and reduced assay time, we have developed an LAL assay approach based on a detection mechanism that is different from those being used in industry, namely, gel-clot, turbidimetric, and chromogenic detection. Using a unique open-microcavity photonic-crystal biosensor to monitor the change in the refractive index due to the reaction between LAL regents and endotoxins, we have demonstrated that this approach has improved the LAL assay sensitivity by 200 times compared with the commercial standard methods, reduced the time needed for the assay by more than half, and eliminated the necessity to incubate the test samples. This study opens up the possibility of using the significantly improved LAL assays for a wide range of applications.
© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE)
Jonathan Scudder, Jing Yong Ye, "<italic<Limulus</italic< amoebocyte lysate test via an open-microcavity optical biosensor," Journal of Biomedical Optics 23(2), 027001 (6 February 2018). https://doi.org/10.1117/1.JBO.23.2.027001 Submission: Received 30 September 2017; Accepted 12 January 2018
Submission: Received 30 September 2017; Accepted 12 January 2018



Optical biosensors


Biological research

In vitro testing

Statistical analysis

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