21 March 2018 Multiscale optical imaging of rare-earth-doped nanocomposites in a small animal model
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Abstract
Rare-earth-doped nanocomposites have appealing optical properties for use as biomedical contrast agents, but few systems exist for imaging these materials. We describe the design and characterization of (i) a preclinical system for whole animal in vivo imaging and (ii) an integrated optical coherence tomography/confocal microscopy system for high-resolution imaging of ex vivo tissues. We demonstrate these systems by administering erbium-doped nanocomposites to a murine model of metastatic breast cancer. Short-wave infrared emissions were detected in vivo and in whole organ imaging ex vivo. Visible upconversion emissions and tissue autofluorescence were imaged in biopsy specimens, alongside optical coherence tomography imaging of tissue microstructure. We anticipate that this work will provide guidance for researchers seeking to image these nanomaterials across a wide range of biological models.
© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE)
Laura M. Higgins, Vidya Ganapathy, Harini Kantamneni, Xinyu Zhao, Yang Sheng, Mei Chee Tan, Charles M. Roth, Richard E. Riman, Prabhas V. Moghe, Mark C. Pierce, "Multiscale optical imaging of rare-earth-doped nanocomposites in a small animal model," Journal of Biomedical Optics 23(3), 030505 (21 March 2018). https://doi.org/10.1117/1.JBO.23.3.030505 . Submission: Received: 28 September 2017; Accepted: 26 February 2018
Received: 28 September 2017; Accepted: 26 February 2018; Published: 21 March 2018
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