15 March 2018 High-throughput ultraviolet photoacoustic microscopy with multifocal excitation
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Abstract
Ultraviolet photoacoustic microscopy (UV-PAM) is a promising intraoperative tool for surgical margin assessment (SMA), one that can provide label-free histology-like images with high resolution. In this study, using a microlens array and a one-dimensional (1-D) array ultrasonic transducer, we developed a high-throughput multifocal UV-PAM (MF-UV-PAM). Our new system achieved a 1.6  ±  0.2  μm lateral resolution and produced images 40 times faster than the previously developed point-by-point scanning UV-PAM. MF-UV-PAM provided a readily comprehensible photoacoustic image of a mouse brain slice with specific absorption contrast in ∼16  min, highlighting cell nuclei. Individual cell nuclei could be clearly resolved, showing its practical potential for intraoperative SMA.
© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE)
Toru Imai, Junhui Shi, Terence T. W. Wong, Lei Li, Liren Zhu, Lihong V. Wang, "High-throughput ultraviolet photoacoustic microscopy with multifocal excitation," Journal of Biomedical Optics 23(3), 036007 (15 March 2018). https://doi.org/10.1117/1.JBO.23.3.036007 . Submission: Received: 8 December 2017; Accepted: 22 February 2018
Received: 8 December 2017; Accepted: 22 February 2018; Published: 15 March 2018
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