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24 March 2018 In vivo high-resolution cortical imaging with extended-focus optical coherence microscopy in the visible-NIR wavelength range
Paul J. Marchand, Daniel Szlag, Arno Bouwens, Theo Lasser
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Journal of Biomedical Optics, Vol. 23, Issue 3, 036012 (March 2018). https://doi.org/10.1117/1.JBO.23.3.036012
Abstract
Visible light optical coherence tomography has shown great interest in recent years for spectroscopic and high-resolution retinal and cerebral imaging. Here, we present an extended-focus optical coherence microscopy system operating from the visible to the near-infrared wavelength range for high axial and lateral resolution imaging of cortical structures in vivo. The system exploits an ultrabroad illumination spectrum centered in the visible wavelength range (λc  =  650  nm, Δλ  ∼  250  nm) offering a submicron axial resolution (∼0.85  μm in water) and an extended-focus configuration providing a high lateral resolution of ∼1.4  μm maintained over ∼150  μm in depth in water. The system’s axial and lateral resolution are first characterized using phantoms, and its imaging performance is then demonstrated by imaging the vasculature, myelinated axons, and neuronal cells in the first layers of the somatosensory cortex of mice in vivo.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Paul J. Marchand, Daniel Szlag, Arno Bouwens, and Theo Lasser "In vivo high-resolution cortical imaging with extended-focus optical coherence microscopy in the visible-NIR wavelength range," Journal of Biomedical Optics 23(3), 036012 (24 March 2018). https://doi.org/10.1117/1.JBO.23.3.036012
Received: 14 January 2018; Accepted: 7 March 2018; Published: 24 March 2018
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Cited by 6 scholarly publications.
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KEYWORDS
Imaging systems
In vivo imaging
Image resolution
Axons
Optical coherence tomography
Backscatter
Optical coherence microscopy
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