24 April 2018 Widefield and total internal reflection fluorescent structured illumination microscopy with scanning galvo mirrors
Author Affiliations +
Abstract
We present an alternative approach to realize structured illumination microscopy (SIM), which is capable for live cell imaging. The prototype utilizes two sets of scanning galvo mirrors, a polarization converter and a piezo-platform to generate a fast shifted, s-polarization interfered and periodic variable illumination patterns. By changing the angle of the scanning galvanometer, we can change the position of the spots at the pupil plane of the objective lens arbitrarily, making it easy to switch between widefield and total internal reflection fluorescent-SIM mode and adapting the penetration depth in the sample. Also, a twofold resolution improvement is achieved in our experiments. The prototype offers more flexibility of pattern period and illumination orientation changing than previous systems.
© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE)
Youhua Chen, Youhua Chen, Ruizhi Cao, Ruizhi Cao, Wenjie Liu, Wenjie Liu, Dazhao Zhu, Dazhao Zhu, Zhiming Zhang, Zhiming Zhang, Cuifang Kuang, Cuifang Kuang, Xu Liu, Xu Liu, } "Widefield and total internal reflection fluorescent structured illumination microscopy with scanning galvo mirrors," Journal of Biomedical Optics 23(4), 046007 (24 April 2018). https://doi.org/10.1117/1.JBO.23.4.046007 . Submission: Received: 17 January 2018; Accepted: 3 April 2018
Received: 17 January 2018; Accepted: 3 April 2018; Published: 24 April 2018
JOURNAL ARTICLE
9 PAGES


SHARE
Back to Top