8 May 2018 Numerical modeling of two-photon focal modulation microscopy with a sinusoidal phase filter
Author Affiliations +
Abstract
A spatiotemporal phase modulator (STPM) is theoretically investigated using the vectorial diffraction theory. The STPM is equivalent to a time-dependent phase-only pupil filter that alternates between a homogeneous filter and a stripe-shaped filter with a sinusoidal phase distribution. It is found that two-photon focal modulation microscopy (TPFMM) using this STPM can significantly suppress the background contribution from out-of-focus ballistic excitation and achieve almost the same resolution as two-photon microscopy. The modulation depth is also evaluated and a compromise exists between the signal-to-background ratio and signal-to-noise ratio. The theoretical investigations provide important insights into future implementations of TPFMM and its potential to further extend the penetration depth of nonlinear microscopy in imaging multiple-scattering biological tissues.
© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE)
Rui Chen, Rui Chen, Shuhao Shen, Shuhao Shen, Nanguang Chen, Nanguang Chen, } "Numerical modeling of two-photon focal modulation microscopy with a sinusoidal phase filter," Journal of Biomedical Optics 23(5), 055002 (8 May 2018). https://doi.org/10.1117/1.JBO.23.5.055002 . Submission: Received: 30 November 2017; Accepted: 19 April 2018
Received: 30 November 2017; Accepted: 19 April 2018; Published: 8 May 2018
JOURNAL ARTICLE
8 PAGES


SHARE
Back to Top