Laser trap ionization for identification of human erythrocytes with variable hemoglobin quantitation

Michele Kelley; James Cooper; Daniel Devito; Robert Mushi; Maria del Pilar Aguinaga; Daniel B. Erenso

doi:10.1117/1.JBO.23.5.055005

30 May 2018 Laser trap ionization for identification of human erythrocytes with variable hemoglobin quantitation

Michele Kelley, James Cooper, Daniel Devito, Robert Mushi, Maria del Pilar Aguinaga, Daniel B. Erenso

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Journal of Biomedical Optics, Vol. 23, Issue 5, 055005 (May 2018). https://doi.org/10.1117/1.JBO.23.5.055005

Abstract

An approach to an established technique that is potentially applicable for a more comprehensive understanding of the electrical properties of red blood cells (RBCs) is presented. Using a high-intensity gradient laser trap, RBCs can be singly trapped and consequentially ionized. The subsequent dynamics of the ionized cell allows one to calculate the charge developed and the ionization energy (IE) through a Newtonian-based analysis. RBCs with two different hemoglobin (Hb) types were ionized. The first sample was identified as carrying Hb HbAA (normal Hb) and the second one was identified as carrying HbAC (HbC trait). By analyzing the charge developed on each cell and several other related factors, we were able to discern a difference between the main Hb types contained within the individual RBC, independent of cell size. A relationship between the charge developed and the IE of the cell was also established based on the electrical properties of RBCs. Thus, we present this laser trapping technique as a study of the electrical properties of RBCs and as possible biomedical tool to be used for the differentiation of Hb types.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Michele Kelley, James Cooper, Daniel Devito, Robert Mushi, Maria del Pilar Aguinaga, and Daniel B. Erenso "Laser trap ionization for identification of human erythrocytes with variable hemoglobin quantitation," Journal of Biomedical Optics 23(5), 055005 (30 May 2018). https://doi.org/10.1117/1.JBO.23.5.055005

Received: 19 February 2018; Accepted: 16 May 2018; Published: 30 May 2018

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