Simultaneous in vivo confocal reflectance and two-photon retinal ganglion cell imaging based on a hollow core fiber platform

Tschackad Kamali; Jörg Fischer; Spring Farrell; William H. Baldridge; Gerhard Zinser; Balwantray C. Chauhan

doi:10.1117/1.JBO.23.9.091405

26 March 2018 Simultaneous in vivo confocal reflectance and two-photon retinal ganglion cell imaging based on a hollow core fiber platform

Tschackad Kamali, Jörg Fischer, Spring Farrell, William H. Baldridge, Gerhard Zinser, Balwantray C. Chauhan

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Journal of Biomedical Optics, Vol. 23, Issue 9, 091405 (March 2018). https://doi.org/10.1117/1.JBO.23.9.091405

Abstract

We have developed a compact hollow core fiber (HCF)-based imaging platform capable of simultaneous in vivo confocal reflectance and two-photon imaging through the mouse pupil. We demonstrate the performance of this platform by imaging retinal ganglion cells (RGCs) in which the fluorophores YFP and GCaMP3 are expressed in Thy1-YFP-16 and Thy1-GCaMP3 transgenic mice, respectively. Confocal reflectance images of the mouse retina served as a reference for the simultaneous acquisition of the two-photon signals that clearly showed RGCs with single-cell resolution. The use of an HCF platform makes the system compact with future application in the longitudinal investigation into the structure and function of healthy and diseased RGCs.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Tschackad Kamali, Jörg Fischer, Spring Farrell, William H. Baldridge, Gerhard Zinser, and Balwantray C. Chauhan "Simultaneous in vivo confocal reflectance and two-photon retinal ganglion cell imaging based on a hollow core fiber platform," Journal of Biomedical Optics 23(9), 091405 (26 March 2018). https://doi.org/10.1117/1.JBO.23.9.091405

Received: 28 November 2017; Accepted: 5 March 2018; Published: 26 March 2018

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