4 September 2018 Early detection of virus infection in live human cells using Raman spectroscopy
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Abstract
Virus infection of a human cell was determined only 3 h after invagination. We used viral vector Ad-CMV-control (AdC), which lacks the E1 gene coding for early polypeptide 1 (E1). AdC can replicate in human embryonic kidney 293 (HEK293) cells into which the E1 gene has been transfected. According to partial least-square regression discriminant analysis, it was assumed that two kinds of reaction take place in the cell during viral invasion. The first response of the cell was determined 3 h after the virus invasion, and the second one was determined ∼9  h later. The first one seems to be due to compositional changes in DNA. Analysis of large-scale datasets strongly indicated that the second reaction can be attributed to a reduction in protein concentration or uptake of phenylalanine into the nucleus.
© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE)
Kamila Moor, Kamila Moor, Yusuke Terada, Yusuke Terada, Akinori Taketani, Akinori Taketani, Matsuyoshi Hiroko, Matsuyoshi Hiroko, Kiyoshi Ohtani, Kiyoshi Ohtani, Hidetoshi Sato, Hidetoshi Sato, "Early detection of virus infection in live human cells using Raman spectroscopy," Journal of Biomedical Optics 23(9), 097001 (4 September 2018). https://doi.org/10.1117/1.JBO.23.9.097001 . Submission: Received: 20 March 2018; Accepted: 6 August 2018
Received: 20 March 2018; Accepted: 6 August 2018; Published: 4 September 2018
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