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13 March 2020 Spontaneous loss versus stimulation gain in pump-probe microscopy: a proof of concept demonstration
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Abstract

Significance: The large background, narrow dynamic range, and detector saturation have been the common limiting factors in stimulated emission (SE)-based pump-probe microscopy, attributed to the very small signal overriding the very intense laser probe beam. To better differentiate the signal of interest from the background, lock-in detection is used to measure the fluorescence quenching, which is termed spontaneous loss (SL). The advantages are manifold. The spontaneous fluorescence signal can be well separated from both the pump and the probe beams with filters, thus eliminating the background, enlarging the dynamic range, and avoiding the saturation of the detector.

Aim: We propose and demonstrate an integrated pump-probe microscopy technique based on lock-in detection for background removal and dynamic range enhancement through SL detection.

Approach: The experimental setup is configured with a pulsed diode laser at a wavelength λpu  =  635  nm, acting as a pump (excitation) and a mode-locked Ti:sapphire laser at a central wavelength λpr  =  780  nm, serving as the probe beam (stimulation). Both pulse trains are temporally synchronized through high precision delay control by adjusting the length of the triggering cables. The pump and probe beams are alternatively modulated at different frequencies f1 and f2 to extract the stimulated gain (SG) and SL signal.

Results: SG signal shows saturation due to the irradiation of the intense probe beam onto the photodetector. However, the detector saturation does not occur at high probe beam power for SL detection. The fluorescence lifetime images are acquired with reduced background. The theoretical signal-to-noise ratios for SG and SL are also estimated by photon statistics.

Conclusion: We have confirmed that the detection of SL allows the elimination of the large background without photodetector saturation, which commonly exists in SG configuration. This modality would allow unprecedented manipulation and investigation of fluorophores in fluorescence imaging.

1.

Introduction

Pump-probe microscopy has been a versatile and powerful platform that takes advantage of transient absorption (TA) and gain in nonlinear optical processes with many imaging modalities, including both labeled and label-free ones. The labeled imaging modalities, such as stimulated emission (SE), excited state absorption, and ground state depletion, are able to reveal molecular specificity, improve resolution, and enhance penetration depth.15 The label-free ones include stimulated Raman scattering (SRS)6 and TA.7 These imaging modalities have been shown to reveal the structural features and transient phenomena in biology and chemistry at picosecond or femtosecond time scales.812 In the pump-probe technique, the pump pulse is used to excite the sample and the induced changes are then monitored by the synchronized probe pulse. Notably, lock-in detection is used throughout the pump-probe microscopy for both labeled and label-free imaging to recover the relatively small modulated signals from the very large background.

Among the above-mentioned modalities, SE is one of the most versatile techniques for scanning optical microscopy, with its renowned application in stimulated emission depletion (STED) microscopy to allow spatial resolution far beyond the diffraction limit.13 In STED microscopy, a common approach is to excite the electrons from ground states to excited states and another laser beam at a wavelength that partially overlaps the emission spectrum of the fluorophore is then used to turn the excited fluorophores to a nonfluorescent state (dark state) by SE. In this way, SE increases the number of photons (gain) in the probe beam (stimulated gain, SG), while it also quenches the fluorescence emission process, known as spontaneous loss (SL). In this case, the rate equations for populations of the ground state (S0) and of the excited state (S1) can be written as14

Eq. (1)

dS0dt=kexcS0+(kfl+kSTED)S1,

Eq. (2)

dS1dt=kexcS0(kfl+kSTED)S1,
where kexc=σabsIexc, kfl=1/τ, and kSTED=σSTEDISTED are the rate of excitation caused by the pump beam, rate of spontaneous emission, and rate of SE caused by the STED beam, respectively. Therefore, increasing the STED beam would shorten the excited state lifetime, τ=1kfl+kSTED. This feature of STED with a low intensity STED beam has been used to improve image resolution.14 Note that the transitions of SE take place in both real states (fluorescence) and virtual states (SRS). In SRS, the pump and Stokes beams are illuminated on the sample when the frequency difference between the pump and the Stokes matches the specific molecular vibrational frequency of a chemical bond. As a result, the Stokes beam experiences photon gain (stimulated Raman gain). On the other hand, the pump beam experiences photon loss (stimulated Raman loss).

In addition, SE-based pump-probe microscopy was carried out for undetectable fluorophores detection,15 subdiffraction fluorescence lifetime imaging,16 and background-free fluorescence imaging.17 SE is also a two-photon process working through real state transition, which has an equivalent cross section several orders of magnitude greater than the virtual ones. Note that in scanning optical microscopy, the penetration depth and signal-to-background ratio are two key advantages claimed by two-photon (2P) excitation due to the nonlinear intensity dependence of the absorption. SE reduced fluorescence microscopy was demonstrated to extend the fundamental depth limit of 2P fluorescence imaging.18 A femtosecond laser is often required to achieve effective excitation efficiency since the transition is through virtual states, which renders such an imaging system being costly, bulky, and complex. In comparison, SE could realize the 2P process with the use of gain-switched laser diodes, greatly reducing the cost and the complexity of operating a femtosecond laser.

In this paper, we are presenting an integrated pump-probe microscopy setup for the detection of both the SG and the SL. Critically, SL detection allows the reduction of high background in the signal and more flexibility in selecting detectors and the corresponding electronics for signal processing. A comparison between SG and SL is highlighted in Table 1.

Table 1

Basic differences between SG and SL.

ComparisonSGSL
CharacteristicsForward emission4-π (epi) emission
Background and noiseLarge background from the probe laserLaser background free with minute shot noise from spontaneous emission
Detection techniqueHeterodyne technique: the modulation transferred signal to the probe beam is extracted by lock-in amplifierBoth heterodyne and gated photon counting [with use time-correlated single-photon counting (TCSPC)] can be used
Working rangeLimited by the saturation level of the photodetectorLimited by the dynamic range of the detector
Amplification and gainUsually there is no gain for the detector usedAmplification is allowed

2.

Experimental

2.1.

Working Principle of Lock-In Detection for Signal Extraction from Stimulated Gain and Spontaneous Loss

The working principle of modulation transfer that carries the SE signal in pump-probe microscopy for SG and SL is shown in Fig. 1. The pump and the probe beams are alternatively modulated at a selected frequency, f, to extract SG and SL, respectively.

Fig. 1

Working principle of modulation transfer for (a) SG and (b) SL in pump-probe microscopy. Either the pump beam or the probe beam is modulated for SG or SL detection. The SG (ISG) and SL (ISL) signals can be extracted by demodulating the probe (IPr) beam and the spontaneous emission accordingly.

JBO_25_3_036501_f001.png

For SG detection, the sample is irradiated with the modulated pump beam and SG is then detected by demodulating the probe beam at the same frequency by a lock-in amplifier. In the same way, when the sample is excited with the unmodulated pump and the modulated probe beam, the loss in spontaneous emission due to SE is extracted by demodulation with the modulation frequency (on the probe beam).

2.2.

Spectral Detection Scheme for Both Stimulated Gain and Spontaneous Loss

The versatile and robust ATTO 647N fluorescent dye (ATTO 647N, ATTO-TEC, Germany) is used for demonstration. The absorption and fluorescence emission spectra of the red fluorescent dye along with the pump (excitation) and probe (stimulated) laser beams are shown in Fig. 2. The pump (λpu=635  nm) and the probe (λpr=780  nm) beams are selected at two different wavelengths that match well with the absorption and emission band spectra of the dye. The spectral filter sets are used in the experiment for two functions: (i) blocking the pump beam completely and allowing the probe beam only that carries SE signal and (ii) blocking both the pump and the probe beams and allowing only fluorescence emission. Critically, in the detection of SL, a band pass filter (marked by green) is used to reject both the pump and the probe wavelengths and pass only the spontaneous emission. While for the detection of SG, a band pass filter (marked by blue) is used to block the pump beam completely and pass the probe beam along with the florescence emission (which is a substantial source of background in SG detection).

Fig. 2

Spectral distribution of the absorption and emission spectrum of ATTO 647N dye and the laser (pump and probe) beams. The SG and SL signals are separately detected at two different channels using appropriate band pass filters.

JBO_25_3_036501_f002.png

2.3.

Optical Microscope Setup

The schematic of the experimental setup for the SG and the SL is shown in Fig. 3. Our pump-probe microscope is configured with a pulse diode laser (LDH-D-C-635M, PicoQuant, Germany) with the pump (excitation) beam at a wavelength of λpu=635  nm and a pulse width of 120  ps, which is synchronized with the mode-locked Ti-sapphire laser (Mira F-900, Coherent Inc.), serving as the probe beam operated at a central wavelength of λpr=780  nm through a trigger diode (TDA200A, PicoQuant, Germany). The maximum pump and probe beams power are set at 2.6 and 50 mW accordingly. The time delay (τ) between the pump and probe pulses are precisely controlled by adjusting the length of the triggering cable and setting the nanosecond delay box (Ortec 425A, Ametek). Two laser line filters (FL635-10 and FL780-10, Thorlabs) are used to remove the unwanted wavelengths that are associated with the pump and probe beams. The probe beam pulses (200 fs) are passed through two 15-cm long dispersive glass rods (SF-6) for pulse width stretch (to 2.6  ps) to avoid 2P excitation. An anamorphic prism (PS875-A, Thorlabs) is used to transform the elliptical mode of the pump beam into a circular one for better mode matching and tighter focusing. Both beams are coupled into a laser scanning unit (FV300, Olympus, Japan) through a dichroic mirror (FF01-720/SP, Semrock). The combined beams are focused onto the sample by an objective lens (UPlanFL 10X 0.30, Olympus, Japan) and the transmitted light is collected by another objective lens (UPlanFL 10X 0.30, Olympus, Japan). For SG, the pump beam is modulated with a lock-in amplifier (HF2LI, Zurich Instrument, Switzerland) at the frequency of 100 KHz. A bandpass filter (FF01-769/41-25, Semrock) is used to block the pump beam completely and let only the probe beam along with some fluorescence to pass through the filter. The SE photons gained by the probe beam propagating in the transmission direction are detected by a silicon photodiode (PDA36A, Thorlabs). For comparison, in SL, the probe beam is modulated with an electro-optic modulator (M350-80LA, Conoptics Inc.) also at the frequency of 100 KHz. The spontaneous emission is reflected in the backscattered direction by a dichroic mirror (ZT685dcrb, Chroma Technology). A bandpass filter (FF01-700/13-25, Semrock) is placed before the photomultiplier tube (PMT) to completely block the pump and the probe beams. The SL signal is detected by demodulating the output of the PMT (R376, Hamamatsu, Japan) with the lock-in amplifier. The time constant of the lock-in amplifier is set at 2 ms. The output of the lock-in amplifier is connected to the analog-to-digital channel of the scanning unit to reconstruct the images. For SG and SL signals’ measurements, the fluorescent ATTO 647N dye is dissolved in deionized water with various concentrations (0.1 to 1 mM). The dye solution is injected into the microchannel slide (15μ-slide, ibidi GmbH, Germany) for testing. A piece of lens cleaning tissue paper is immersed inside the ATTO 647N dye solution and sandwiched between two cover glass slides for time-resolved imaging.

Fig. 3

Experimental setup of the pump-probe microscopy for the SG and the SL. EOM, electro-optic modulator; LLF, laser line filter; M, mirror; DM, dichroic mirror; BS, beam splitter; OL, objective lens; S, sample; CL, condenser lens; L, lens; F, bandpass filter; PD, photodiode; PMT, photomultiplier tube. The backscattered scheme is used to detect the SL whereas the SG is detected in transmission mode.

JBO_25_3_036501_f003.png

3.

Results and Discussions

Figure 4(a) shows the SG signal as a function of the probe beam power. When the probe laser power reaches 3.5 mW, the SG signal starts to show saturation due to the intense power of the probe beam on the photodetector.

Fig. 4

The (a) SG and (b) SL signals as a function of the probe beam power. The photodetector saturation effect is marked with a red dashed oval. The pump beam power is fixed at 2.6 mW. Inset: SL signal as a function of various dye concentrations (0.1 to 1 mM).

JBO_25_3_036501_f004.png

In such a high NA setting, the SG contains a large background, which is attributed to the spontaneous emission caused by the pump beam. Notably, the SE is also the dominant fluorescence quenching process with the wavelength-dependent SE cross section. 19 The SG and the SL are thus correlated with each other. The fluorescence reduction rate of a single molecule can be described as

Eq. (3)

RSERF=RR=frepkexcτexcηkSEkfl+kSE,
where RSERF, frep, kexc, τexc, η, kSE, and kfl are the fluorescence reduction rate, repetition rate, excitation rate, pulse width, fluorescence quantum yield, SE rate, and fluorescence emission rate, respectively.18 In SG measurements, some spontaneous fluorescence signal (forming the background) is always present along with the SE signal and cannot be filtered out since the probe beam lies within the emission band of the fluorescent dye. For comparison, Fig. 4(b) shows the SL signal increases linearly with the probe beam power. The SL signal does not saturate at a high laser power of 50  mW, and the detected SL dose not contribute to any background or detector saturation.

The SL signal as a function of time delay between two pulses is shown in Fig. 5(a). In a fluorescence quenching experiment, the fluorescence intensity and relative delay are given as

Eq. (4)

I0II=qexp(td/τ),
where I0 and I are the intensities in the absence and presence of SE pulses, q is the extent of quenching, td is the relative time delay between the pump and probe pulses, and τ is the fluorescence lifetime of the fluorophore.20 The fluorescence lifetime images with different delay times are shown in Fig. 5(b). When compared with the data acquired in our previous work,21 the lifetime images are obtained with greatly reduced backgrounds.

Fig. 5

(a) SL signal as a function of the relative time delay between the pump and the probe pulses. (b) The fluorescence lifetime images of lens cleaning tissue paper at different delay times. Note that all the color coded images are acquired at 512×512  pixels with a scale bar of 100  μm.

JBO_25_3_036501_f005.png

In SG detection, the probe beam carrying the SE signal directly irradiates the photodetector and contributes substantial noise due to its high intensity and fluctuation. The noise level due to the probe beam is evaluated by switching off the pump beam. The root-mean-square noise, which is the standard deviation of the detected signal over a period of time, is measured as the input power of the probe beam, as shown in Fig. 6. The experimental result is compared with the theoretical shot noise level, which is evaluated by Eq. (5) with the following parameters for the photodetector: gain, G=0.75×103  V/A, elementary charge, q=1.6×1019  C, responsivity (r)=0.49  A/W at 780 nm, and optical power, P.

Eq. (5)

vshot=G2qrP.
In this condition, the shot noise level is limited at a very low optical probe power (110 to 160  μW). As the power of the probe beam increases, the main source of noise is switched to the laser noise. However, the laser intensity noise does not affect the SL signal except for shot noise.

Fig. 6

Noise level of the lock-in signal as a function of optical power of the probe beam. The triangle (red) and square (green) represent the measured noise level and theoretical shot noise, respectively.

JBO_25_3_036501_f006.png

In an optical imaging system, signal-to-noise ratio (SNR) is a critical parameter, which is also estimated by theoretical calculation. Here, we used Poisson statistics to evaluate the theoretical SNRs of the SG and SL. The experimental parameters for theoretical calculation are listed in Table 2.

Table 2

Experimental parameters for theoretical SNR calculation.

ParameterValue
Laser repetition frequency (frep)76 MHz
Pump wavelength (λpu)635 nm
Probe wavelength (λpr)780 nm
Pump power (Ppu)2.6 mW
Probe power (Ppr)3.6 mW
Pump photons per pulse (npu/pulse)1.09×108
Pump airy disc diameter (dpu)2.58×106  m
Pump axial resolution (zpu)9.38×106  m
Pump beam waist area (Apu)5.23×1012  m2
Probe photons per pulse (npr/pulse)1.86×108
Probe airy disc diameter (dpr)3.17×106m
Probe axial resolution (zpr)1.15×105  m
Probe beam waist area (Apr)7.89×1012  m2
Concentration (C)1 mM
Avogadro’s number (NA)6.02×1023  mol1
Speed of light (c)3×108  m/s
Planck’s constant (h)6.626×1034  Js
Numerical aperture (N.A)0.3
Bandpass filter efficiency23%
Refractive index (η)1.33
Quantum efficiency of ATTO 647N (ηfl)65%
Absorption cross-section (σabs)2.5×1020  m2
SE cross-section (σS.E.)5×1021  m2
Lifetime of (τfl)3.5 ns
Probability of SE (kS.E.kfl+kS.E.)0.058
Solid angle at N.A = 0.3 (Ω)0.0128
No. of excited molecules per area (CNAzpu)5.64×1018  m2
No. of stimulated molecules per area (CNAzpunpu/pulseApuσabs)2.93×1018  m2

The number of spontaneous photons per pulse in the pump beam waist is estimated as

(No. of excited molecules/area)×(npu/pulse)×σabs×Ω×ηfl=1.27×105.

Similarly, the number of SE photons per pulse in the probe beam waist is then

(No. of stimulated molecules/area)×σS.E.×(npr/pulse)×(kS.E.kfl+kS.E.)×filter efficiency=3.63×104.

The SNR of SG can be estimated as

SNR=SEsignal{(SEsignal)+(Spontaneous emission)},SNR=(3.63×104){(3.63×104)+(1.27×105)},SNR=89.

The SNR of SG is limited by the photodetector saturation window due to the strong probe beam power.

The SNR of SL can be calculated as

SNR=Spontaneous signalSponatenous signal,SNR=(1.27×105)(1.27×105),SNR=356.

In SL, the selection of a high gain detector, such as a PMT, would improve the SNR.

As discussed above, the benefits and limitations of SL are illustrated using conventional analog lock-in detection mode. Notably, the analog measurements are always affected by the detector’s gain [such as a photodiode (PD) and PMT] and the electronics noise (such as thermal noise, flicker noise, and shot noise). For a sine wave modulation, the effective count rate (rT) over a bin time (T) can be expressed as22

Eq. (6)

rT(t)=r0+A[sin(πfmT)πfmT]sin(2πfmt+πfmT+θ),
where r0 is a constant rate, A is the modulation depth, and fm is the modulation frequency. Therefore, a high count rate can be obtained with a longer bin time. The gated photon counting approach can also be used to increase the count rate with a short gating interval to provide high detection efficiency and SNR.

Note that the lock-in amplifier extracts the SL signal from an extremely noisy background. The electronic noise is one of the predominant noises for lock-in detection. In addition, lock-in detection is analog signal processing in nature and is more susceptible to noise and signal distortion. Alternatively, digitally detecting the signal through photon counting will greatly improve the electronic noise and the reliability. The gated photon counting can be an advantageous alternative for lock-in detection under the pump-probe scheme.12 Notably, the lifetime of the fluorescence dye is in the nanosecond time regime, which allows synchronization of the pump pulses with the probe pulses at half of the repetition frequency (38  MHz).23 In this way, the highest possible modulation can be achieved and is termed subharmonic synchronization.

4.

Summary and Future Perspective

In summary, we have successfully established a new pump-probe microscopy technique for the detection of SG and SL signals. In addition, detection of SL allows a much wider dynamic range without saturating the detector and the elimination of the large background, which commonly exists in the SE signal. In this way, fluorescence lifetime images can be obtained with greatly reduced backgrounds.

Finally, and critically, our ultimate goal is to insert the pump-probe microscopy system into a fully digital data acquisition scheme based on photon-counting detection. The pump-probe imaging is equivalent to 2P imaging, which provides optical sectioning capability with high contrast. In particular, our technique is expected to investigate fluorophores and improve SNR in pump-probe microscopy.

Disclosures

We have no competing financial interests.

Acknowledgments

The authors would like to acknowledge financial support from the Ministry of Science and Technology, Taiwan (MOST 105-2112-M-010-001-MY3 and MOST 108-2112-M-010-001). The authors thank Sandip Ghosh at National Yang-Ming University, Taipei, Taiwan, for his assistance on characterizing the optical absorption spectrum of the dye used in this study.

References

1. 

T. Dellwig, M. R. Foreman and F.-J. Kao, “Coherent long-distance signal detection using stimulated emission: a feasibility study,” Chin. J. Phys., 48 873 –884 (2010). Google Scholar

2. 

D. Fu et al., “Two-color, two-photon, and excited-state absorption microscopy,” J. Biomed. Opt., 12 054004 (2007). https://doi.org/10.1117/1.2780173 JBOPFO 1083-3668 Google Scholar

3. 

S. Chong, W. Min and X. S. Xie, “Ground-state depletion microscopy: detection sensitivity of single-molecule optical absorption at room temperature,” J. Phys. Chem. Lett., 1 3316 –3322 (2010). https://doi.org/10.1021/jz1014289 JPCLCD 1948-7185 Google Scholar

4. 

M. C. Fischer et al., “Invited review article: pump-probe microscopy,” Rev. Sci. Instrum., 87 031101 (2016). https://doi.org/10.1063/1.4943211 RSINAK 0034-6748 Google Scholar

5. 

J. Märk et al., “Photoacoustic imaging of fluorophores using pump-probe excitation,” Biomed. Opti. Express, 6 2522 –2535 (2015). https://doi.org/10.1364/boe.6.002522 Google Scholar

6. 

X. Audier, N. Balla and H. Rigneault, “Pump-probe micro-spectroscopy by means of an ultra-fast acousto-optics delay line,” Opt. Lett., 42 294 –297 (2017). https://doi.org/10.1364/OL.42.000294 OPLEDP 0146-9592 Google Scholar

7. 

C. Y. Wong et al., “Revealing exciton dynamics in a small-molecule organic semiconducting film with subdomain transient absorption microscopy,” J. Phys. Chem. C, 117 22111 –22122 (2013). https://doi.org/10.1021/jp407645k JPCCCK 1932-7447 Google Scholar

8. 

E. M. Grumstrup et al., “Pump-probe microscopy: visualization and spectroscopy of ultrafast dynamics at the nanoscale,” Chem. Phys., 458 30 –40 (2015). https://doi.org/10.1016/j.chemphys.2015.07.006 CMPHC2 0301-0104 Google Scholar

9. 

E. S. Massaro, A. H. Hill and E. M. Grumstrup, “Super-resolution structured pump-probe microscopy,” ACS Photonics, 3 501 –506 (2016). https://doi.org/10.1021/acsphotonics.6b00140 Google Scholar

10. 

J. Märk, F.-J. Schmitt and J. Laufer, “Photoacoustic imaging of the excited state lifetime of fluorophores,” J. Opt., 18 054009 (2016). https://doi.org/10.1088/2040-8978/18/5/054009 Google Scholar

11. 

K.-H. Lin et al., “Phonon dynamics of single nanoparticles studied using confocal pump-probe backscattering,” Appl. Phys. Lett., 113 171906 (2018). https://doi.org/10.1063/1.5048669 APPLAB 0003-6951 Google Scholar

12. 

T. Fujiwara, “Time-resolved single-molecule fluorescence microscopy: pump-probe scheme employing bursts of pulses and gated photon counting,” Opt. Commun., 420 215 –218 (2018). https://doi.org/10.1016/j.optcom.2018.03.058 OPCOB8 0030-4018 Google Scholar

13. 

S. W. Hell and J. Wichmann, “Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy,” Opt. Lett., 19 780 –782 (1994). https://doi.org/10.1364/OL.19.000780 OPLEDP 0146-9592 Google Scholar

14. 

G. Vicidomini et al., “Sharper low-power STED nanoscopy by time gating,” Nat. Methods, 8 571 –573 (2011). https://doi.org/10.1038/nmeth.1624 1548-7091 Google Scholar

15. 

W. Min et al., “Imaging chromophores with undetectable fluorescence by stimulated emission microscopy,” Nature, 461 1105 –1109 (2009). https://doi.org/10.1038/nature08438 Google Scholar

16. 

P.-Y. Lin et al., “Fluorescence lifetime imaging microscopy with subdiffraction-limited resolution,” Jpn. J. Appl. Phys., 52 028004 (2013). https://doi.org/10.7567/JJAP.52.028004 Google Scholar

17. 

S. Das et al., “Background free imaging in stimulated emission fluorescence microscopy,” J. Opt., 21 125301 (2019). https://doi.org/10.1088/2040-8986/ab4dc2 Google Scholar

18. 

L. Wei, Z. Chen and W. Min, “Stimulated emission reduced fluorescence microscopy: a concept for extending the fundamental depth limit of two-photon fluorescence imaging,” Biomed. Opt. Express, 3 1465 –1475 (2012). https://doi.org/10.1364/BOE.3.001465 BOEICL 2156-7085 Google Scholar

19. 

E. Rittweger et al., “Fluorescence depletion mechanisms in super-resolving STED microscopy,” Chem. Phys. Lett., 442 483 –487 (2007). https://doi.org/10.1016/j.cplett.2007.06.017 CHPLBC 0009-2614 Google Scholar

20. 

I. Gryczynski, S. W. Hell and J. R. Lakowicz, “Light quenching of pyridine2 fluorescence with time-delayed pulses,” Biophys. Chem., 66 13 –24 (1997). https://doi.org/10.1016/S0301-4622(96)02264-8 BICIAZ 0301-4622 Google Scholar

21. 

P.-Y. Lin et al., “Long working distance fluorescence lifetime imaging with stimulated emission and electronic time delay,” Opt. Express, 20 11445 –11450 (2012). https://doi.org/10.1364/OE.20.011445 OPEXFF 1094-4087 Google Scholar

22. 

S. Fray et al., “Photon-counting distributions of modulated laser beams,” Phys. Rev., 153 357 –359 (1967). https://doi.org/10.1103/PhysRev.153.357 PHRVAO 0031-899X Google Scholar

23. 

S. Das et al., “Synchronized subharmonic modulation in stimulated emission microscopy,” Opt. Express, 27 27159 –27167 (2019). https://doi.org/10.1364/OE.27.027159 OPEXFF 1094-4087 Google Scholar

Biography

Subir Das is currently a postdoctoral fellow at the Institute of Biophotonics, National Yang-Ming University, Taiwan. He received his BSc and MSc degrees in physics and nanoscience and technology from Gauhati University in 2011 and Tezpur University in 2013, India, respectively. He received his PhD in biophotonics from the Institute of Biophotonics, National Yang-Ming University, Taiwan, in 2019. His current research interests include advanced optical microscopy, optical beam-induced current microscopy, and ultrasound imaging. He was the president of the OSA student chapter at National Yang-Ming University from 2017 to 2018. He is a member of SPIE, OSA, and IEEE.

Khalil Ur Rehman is currently pursuing his PhD at the Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan, under the supervision of Professor Fu-Jen Kao. He received his MPhil degree in applied physics from the Centre for Advanced Studies in Physics, Government College University, Lahore, Pakistan, in 2015. He has expertise in light–matter interactions, fluorescence lifetime imaging, and fluorescence resonance energy transfer. His current research in the field of polarization-resolved based stimulated emission pump-probe microscopy.

Guan-Yu Zhuo is now an assistant professor in the Institute of New Drug Development, China Medical University (CMU). He directs the Biophotonics Laboratory at CMU for advanced science and optical imaging technology. He received his BS and MS degrees in physics from the National Chung Cheng University, Taiwan, in 2005 and 2007, respectively. He received his PhD in physics from the National Taiwan University in 2012. He studied high-end microscopy techniques with Professor Fu-Jen Kao for more than 5 years.

Fu-Jen Kao received his BA degree from the National Taiwan University in 1983 and his MA and PhD degrees from Cornell University, in 1988 and 1993, respectively, all in physics. He is currently a professor at the Institute of Biophotonics, National Yang-Ming University, Taiwan. He was also the former director of the Institute of Biophotonics, National Yang-Ming University, and the former president of the Physics Society of ROC, Taiwan. He was the vice president of the Association of Asia Pacific Physical Societies from 2017 to 2019. In addition, he has served as the chief of research and planning, Office of Research Affairs, a professor at the Institute of Electro-Optical Engineering, and a professor at the Department of Physics, National Sun Yat-sen University. The research laboratory led by him has successfully developed many advanced techniques based on multiphoton microscopy with a wide variety of imaging modalities, including two-photon, OBIC, SHG, THG, CARS, stimulated emission, FLIM/FRET, etc. In addition to championing these developments, he has transferred many of the above techniques to a large number of interested research groups both domestically and internationally. He has authored more than 100 SCI journal papers, edited two books, and presented his research at more than 100 international conferences. He is a fellow of Royal Microscopy Society, Taiwan Physical Society, and SPIE, and a reviewer of a number of international research journals.

© The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
Subir Das, Khalil Ur Rehman, Guan-Yu Zhuo, and Fu-Jen Kao "Spontaneous loss versus stimulation gain in pump-probe microscopy: a proof of concept demonstration," Journal of Biomedical Optics 25(3), 036501 (13 March 2020). https://doi.org/10.1117/1.JBO.25.3.036501
Received: 24 September 2019; Accepted: 25 February 2020; Published: 13 March 2020
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