1 April 1999 Cell viability and DNA denaturation measurements by two-photon fluorescence excitation in CW Al:GaAs diode laser optical traps
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Abstract
Cell viability and DNA denaturation are measured through two-photon fluorescence excitation using a single diode laser beam as the trapping and exciting source simultaneously. Two-photon fluorescence emission spectra are presented for CHO cells and T lymphocytes loaded with various fluorescent probes. This single beam method is demonstrated to be a safe tool to monitor the viability of optically trapped cells, even under intense 809 nm diode laser illumination (~106 W/cm2). The dynamics of cellular necrosis is monitored by adding ethanol to the cell suspension during trapping. Thermal damage to heat-treated samples is assessed by recording shifts in the emission spectra from trapped cells loaded with the nucleic acid probe, acridine orange.
Zhanxiang Zhang, Gregory J. Sonek, Xunbin Wei, Chung-Ho Sun, Michael W. Berns, Bruce J. Tromberg, "Cell viability and DNA denaturation measurements by two-photon fluorescence excitation in CW Al:GaAs diode laser optical traps," Journal of Biomedical Optics 4(2), (1 April 1999). https://doi.org/10.1117/1.429948 . Submission:
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