1 April 1999 Cell viability and DNA denaturation measurements by two-photon fluorescence excitation in CW Al:GaAs diode laser optical traps
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Abstract
Cell viability and DNA denaturation are measured through two-photon fluorescence excitation using a single diode laser beam as the trapping and exciting source simultaneously. Two-photon fluorescence emission spectra are presented for CHO cells and T lymphocytes loaded with various fluorescent probes. This single beam method is demonstrated to be a safe tool to monitor the viability of optically trapped cells, even under intense 809 nm diode laser illumination (~106 W/cm2). The dynamics of cellular necrosis is monitored by adding ethanol to the cell suspension during trapping. Thermal damage to heat-treated samples is assessed by recording shifts in the emission spectra from trapped cells loaded with the nucleic acid probe, acridine orange.
Zhanxiang Zhang, Zhanxiang Zhang, Gregory J. Sonek, Gregory J. Sonek, Xunbin Wei, Xunbin Wei, Chung-Ho Sun, Chung-Ho Sun, Michael W. Berns, Michael W. Berns, Bruce J. Tromberg, Bruce J. Tromberg, } "Cell viability and DNA denaturation measurements by two-photon fluorescence excitation in CW Al:GaAs diode laser optical traps," Journal of Biomedical Optics 4(2), (1 April 1999). https://doi.org/10.1117/1.429948 . Submission:
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