1 October 1999 Polarization-based sensing of glucose using an oriented reference film
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Abstract
We describe a new approach to glucose sensing using polarization measurements in the presence of a stretchoriented reference film. The method relies on measurement of the polarized emission from the reference film and of a fluorophore which changes intensity in response to glucose. A glucose-sensitive fluorescent signal was provided by the glucose/galactose binding protein from E. coli. This protein was labeled with an environmentally sensitive fluorophore at a single genetically inserted cysteine residue, and displayed decreased fluorescence upon glucose binding. Using the protein and the reference film we observed glucose-sensitive polarization values for micromolar glucose concentrations. This method of polarization-based sensing is generic and can be used for any sensing fluorophore which displays a change in intensity. In principle, one can construct simple and economical devices for this type of glucose measurement.
Joseph R. Lakowicz, Ignacy Gryczynski, Zygmunt Gryczynski, Leah Tolosa, Lisa Randers-Eichhorn, Govind Rao, "Polarization-based sensing of glucose using an oriented reference film," Journal of Biomedical Optics 4(4), (1 October 1999). https://doi.org/10.1117/1.429955 . Submission:
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