1 October 1999 Polarization-based sensing of glucose using an oriented reference film
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Abstract
We describe a new approach to glucose sensing using polarization measurements in the presence of a stretchoriented reference film. The method relies on measurement of the polarized emission from the reference film and of a fluorophore which changes intensity in response to glucose. A glucose-sensitive fluorescent signal was provided by the glucose/galactose binding protein from E. coli. This protein was labeled with an environmentally sensitive fluorophore at a single genetically inserted cysteine residue, and displayed decreased fluorescence upon glucose binding. Using the protein and the reference film we observed glucose-sensitive polarization values for micromolar glucose concentrations. This method of polarization-based sensing is generic and can be used for any sensing fluorophore which displays a change in intensity. In principle, one can construct simple and economical devices for this type of glucose measurement.
Joseph R. Lakowicz, Joseph R. Lakowicz, Ignacy Gryczynski, Ignacy Gryczynski, Zygmunt Gryczynski, Zygmunt Gryczynski, Leah Tolosa, Leah Tolosa, Lisa Randers-Eichhorn, Lisa Randers-Eichhorn, Govind Rao, Govind Rao, } "Polarization-based sensing of glucose using an oriented reference film," Journal of Biomedical Optics 4(4), (1 October 1999). https://doi.org/10.1117/1.429955 . Submission:
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