1 April 2000 Feasibility of field-based light scattering spectroscopy
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J. of Biomedical Optics, 5(2), (2000). doi:10.1117/1.429980
Abstract
Light scattering spectroscopy (LSS) is a new technique capable of accurately measuring the features of nuclei and other cellular organelles in situ. We present the considerations required to implement and interpret field-based detection in LSS, where the scattered electric field is detected interferometrically, and demonstrate that the technique is experimentally feasible. A theoretical formalism for modeling field-based LSS signals based on Mie scattering is presented. Phase-front uniformity is shown to play an important and novel role. Results of heterodyne experiments with polystyrene microspheres that localize LSS signals to a region about 30 µm in axial extent are reported. In addition, differences between field-based LSS and the earlier intensity-based LSS are discussed.
Changhuei Yang, Lev T. Perelman, Adam Wax, Ramachandra R. Dasari, Michael S. Feld, "Feasibility of field-based light scattering spectroscopy," Journal of Biomedical Optics 5(2), (1 April 2000). http://dx.doi.org/10.1117/1.429980
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KEYWORDS
Light scattering

Heterodyning

Scattering

Sensors

Laser beam diagnostics

Mie scattering

Spectroscopy

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