Automated single-cell sorting system based on optical trapping

Sumi C. Grover; Andrei G. Skirtach; Robert C. Gauthier; Chander Prakash Grover

doi:10.1117/1.1333676

1 January 2001 Automated single-cell sorting system based on optical trapping

Sumi C. Grover, Andrei G. Skirtach, Robert C. Gauthier, Chander Prakash Grover

Author Affiliations +

J. of Biomedical Optics, 6(1), (2001). https://doi.org/10.1117/1.1333676

Abstract

We provide a basis for automated single-cell sorting based on optical trapping and manipulation using human peripheral blood as a model system. A counterpropagating dual-beam optical-trapping configuration is shown theoretically and experimentally to be preferred due to a greater ability to manipulate cells in three dimensions. Theoretical analysis performed by simulating the propagation of rays through the region containing an erythrocyte (red blood cell) divided into numerous elements confirms experimental results showing that a trapped erythrocyte orients with its longest axis in the direction of propagation of the beam. The single-cell sorting system includes an image-processing system using thresholding, background subtraction, and edge-enhancement algorithms, which allows for the identification of single cells. Erythrocytes have been identified and manipulated into designated volumes using the automated dual-beam trap. Potential applications of automated single-cell sorting, including the incorporation of molecular biology techniques, are discussed.

Citation Download Citation

Sumi C. Grover, Sumi C. Grover, Andrei G. Skirtach, Andrei G. Skirtach, Robert C. Gauthier, Robert C. Gauthier, Chander Prakash Grover, Chander Prakash Grover, } "Automated single-cell sorting system based on optical trapping," Journal of Biomedical Optics 6(1), (1 January 2001). https://doi.org/10.1117/1.1333676 . Submission:

ACCESS THE FULL ARTICLE

PERSONAL SIGN IN
Full access may be available with your subscription

Email or Username Forgot your username?

Password Forgot your password?

Show

Remember Email/Username on this computer

Remember Password

No SPIE account? Create an account
or Institutional Sign In via Shibboleth

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $20.00

Non-members: $25.00 ADD TO CART

Show All Keywords

Keywords/Phrases

Search In:

Publication Years