1 July 2001 Fluorescence resonance energy transfer microscopy: a mini review
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J. of Biomedical Optics, 6(3), (2001). doi:10.1117/1.1383063
Abstract
Fluorescence resonance energy transfer (FRET) microscopy is a better method than the x-ray diffraction, nuclear magnetic resonance, or electron microscopy for studying the structure and localization of proteins under physiological conditions. In this paper, we describe four different light microscopy techniques to visualize the interactions of the transcription factor CAATT/enhancer binding protein alpha (C/EBPa) in living pituitary cells. In wide-field, confocal, and two-photon microscopy the FRET image provides twodimensional spatial distribution of steady-state protein–protein interactions. The two-photon imaging technique provides a better FRET signal (less bleedthrough and photobleaching) compared to the other two techniques. This information, although valuable, falls short of revealing transient interactions of proteins in real time. The fluorescence lifetime methods allow us to monitor FRET signals at the moment of the protein interactions at a resolution on the order of subnanoseconds, providing high temporal, as well as spatial resolution. This paper will provide a brief review of the above-mentioned FRET techniques.
Ammasi Periasamy, "Fluorescence resonance energy transfer microscopy: a mini review," Journal of Biomedical Optics 6(3), (1 July 2001). http://dx.doi.org/10.1117/1.1383063
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KEYWORDS
Fluorescence resonance energy transfer

Proteins

Microscopy

Confocal microscopy

Luminescence

Molecules

Signal processing

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