1 January 2003 Kinetics of picosecond laser pulse induced charge separation and proton transfer in bacteriorhodopsin
Author Affiliations +
Abstract
Bacteriorhodopsin (BR) films oriented by an electrophoretic method are deposited on a transparent conductive ITO glass. A counterelectrode of copper and gelose gel is used to compose a sandwich-type photodetector with the structure of ITO/BR film/gelose gel/Cu. A single 30-ps laser pulse and a mode-locked pulse train are respectively used to excite the BR photodetector. The ultrafast falling edge and the bipolar response signal are measured by the digital oscilloscope under seven different time ranges. Marquardt nonlinear least squares fitting is used to fit all the experimental data and a good fitting equation is found to describe the kinetic process of the photoelectric signal. Data fitting resolves six exponential components that can be assigned to a seven-step BR photocycle model: BR→K→KL→L→M→N→O→BR. Comparing tests of the BR photodetector with a 100-ps Si PIN photodiode demonstrates that this type of BR photodetector has at least 100-ps response time and can also serve as a fast photoelectric switch.
Baoli Yao, Dalun Xu, Xun Hou, Kunsheng Hu, Aojin Wang, "Kinetics of picosecond laser pulse induced charge separation and proton transfer in bacteriorhodopsin," Journal of Biomedical Optics 8(1), (1 January 2003). https://doi.org/10.1117/1.1527626
JOURNAL ARTICLE
5 PAGES


SHARE
Back to Top