1 July 2003 Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methods
Author Affiliations +
Abstract
Fluorescence lifetime images are obtained with the laser scanning microscope using two methods: the time-correlated single-photon counting method and the frequency-domain method. In the same microscope system, we implement both methods. We perform a comparison of the performance of the two approaches in terms of signal-to-noise ratio (SNR) and the speed of data acquisition. While in our practical implementation the time-correlated single-photon counting technique provides a better SNR for low-intensity images, the frequency-domain method is faster and provides less distortion for bright samples.
© (2003) Society of Photo-Optical Instrumentation Engineers (SPIE)
Enrico Gratton, Enrico Gratton, Sophie Breusegem, Sophie Breusegem, Jason D. B. Sutin, Jason D. B. Sutin, Qiaoaio Ruan, Qiaoaio Ruan, Nicholas P. Barry, Nicholas P. Barry, } "Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methods," Journal of Biomedical Optics 8(3), (1 July 2003). https://doi.org/10.1117/1.1586704 . Submission:
JOURNAL ARTICLE
10 PAGES


SHARE
RELATED CONTENT

High-speed fluorescence lifetime imaging
Proceedings of SPIE (June 20 2004)
Analysis of noise sources in frequency-domain fluorometry
Proceedings of SPIE (March 31 1992)
Fluorescence lifetime imaging module LIMO for CLSM
Proceedings of SPIE (May 09 2001)
Dynamic saturation optical microscopy
Proceedings of SPIE (February 26 2006)

Back to Top