1 July 2003 Mechanisms of membrane potential sensing with second-harmonic generation microscopy
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We characterize the transmembrane voltage response of a novel second-harmonic generation (SHG) marker using a screening protocol with giant unilamellar vesicles. Two mechanisms are found to contribute to the voltage response: (1) an electro-optic-induced alteration of the molecular hyperpolarizability and (2) an electric-field-induced alteration of the degree of molecular alignment. We quantify the relative weights and of these contributions and provide an upper limit to their response time, which is found to be submillisecond. The identification of two voltage response mechanisms leads to new strategies for the molecular design of membrane potential markers.
© (2003) Society of Photo-Optical Instrumentation Engineers (SPIE)
Thomas Pons, Thomas Pons, Laurent Moreaux, Laurent Moreaux, Olivier Mongin, Olivier Mongin, Mireille H. Blanchard-Desce, Mireille H. Blanchard-Desce, Jerome Mertz, Jerome Mertz, } "Mechanisms of membrane potential sensing with second-harmonic generation microscopy," Journal of Biomedical Optics 8(3), (1 July 2003). https://doi.org/10.1117/1.1581871 . Submission:

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