1 July 2003 Quantitative imaging of protein–protein interactions by multiphoton fluorescence lifetime imaging microscopy using a streak camera
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Abstract
Fluorescence lifetime imaging microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein–protein interactions and intracellular physiology. Recent developments in multiphoton FLIM methods are reviewed and a novel multiphoton FLIM system using a streak camera is described. An example of a typical application of the system is provided in which the fluorescence resonance energy transfer between a donor-acceptor pair of fluorescent proteins within a cellular specimen is measured.
© (2003) Society of Photo-Optical Instrumentation Engineers (SPIE)
Ramanujan Venkata Krishnan, Atsushi Masuda, Victoria Frohlich E. Centonze, Brian A. Herman, "Quantitative imaging of protein–protein interactions by multiphoton fluorescence lifetime imaging microscopy using a streak camera," Journal of Biomedical Optics 8(3), (1 July 2003). https://doi.org/10.1117/1.1577574 . Submission:
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