1 January 2004 Digital image capture and quantification of subtle lens opacities in rodents
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A rapid, sensitive, and cost-effective method is reported for the subjective and objective documentation of subtle opacities in lenses of unanesthetized transgenic mice or selenite-injected rat pups as models for cataract formation. Animal eyes were dilated with eye drops and the animal was positioned in front of a Nikon FS2 photo slit lamp. Slit-lamp observations were recorded using a Canon Optura Pi digital video recorder. High-quality images of opacifying lenses were captured from the video and quantified using densitometry at progressive stages of opacification. In mice, targeted genomic deletion of the proteins CP49 (a lens-specific filament) or Six5 (a model for myotonic dystrophy) resulted in subtle cataracts that were easily recorded and quantified using this instrumentation. In rats, the early progressive changes leading to a dense nuclear opacity caused by selenite injection were easily documented using this instrumentation. Low-cost components combined with a conventional slit-lamp ophthalmoscope were used to capture high-quality images of selected stages of cataract formation for quantitative analysis using commercial software.
© (2004) Society of Photo-Optical Instrumentation Engineers (SPIE)
T. M. Seeberger, T. M. Seeberger, Y. Matsumoto, Y. Matsumoto, A. Alizadeh, A. Alizadeh, Paul G. Fitzgerald, Paul G. Fitzgerald, John I. Clark, John I. Clark, } "Digital image capture and quantification of subtle lens opacities in rodents," Journal of Biomedical Optics 9(1), (1 January 2004). https://doi.org/10.1117/1.1630034 . Submission:

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