1 July 2004 Confocal fluorescence microscope with dual-axis architecture and biaxial postobjective scanning
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Abstract
We present a novel confocal microscope that has dual-axis architecture and biaxial postobjective scanning for the collection of fluorescence images from biological specimens. This design uses two low-numerical-aperture lenses to achieve high axial resolution and long working distance, and the scanning mirror located distal to the lenses rotates along the orthogonal axes to produce arc-surface images over a large field of view (FOV). With fiber optic coupling, this microscope can potentially be scaled down to millimeter dimensions via microelectromechanical systems (MEMS) technology. We demonstrate a benchtop prototype with a spatial resolution ≤4.4 μm that collects fluorescence images with a high SNR and a good contrast ratio from specimens expressing GFP. Furthermore, the scanning mechanism produces only small differences in aberrations over the image FOV. These results demonstrate proof of concept of the dual-axis confocal architecture for in vivo molecular and cellular imaging.
© (2004) Society of Photo-Optical Instrumentation Engineers (SPIE)
Thomas D. Wang, Christopher H. Contag, Michael J. Mandella, Ning Y. Chan, Gordon S. Kino, "Confocal fluorescence microscope with dual-axis architecture and biaxial postobjective scanning," Journal of Biomedical Optics 9(4), (1 July 2004). https://doi.org/10.1117/1.1760760 . Submission:
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