1 July 2004 Second-harmonic imaging of cornea after intrastromal femtosecond laser ablation
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J. of Biomedical Optics, 9(4), (2004). doi:10.1117/1.1756919
Nonlinear laser scanning microscopy is widely used for noninvasive imaging in cell biology and tissue physiology. However, multiphoton fluorescence imaging of dense, transparent connective tissue (e.g., cornea) is challenging since sophisticated labeling or slicing is necessary. High-resolution, high-contrast second harmonic generation (SHG) imaging of corneal tissue based on the intrinsic structure of collagen is discussed. The three-dimensional corneal ultrastructure in depths up to hundreds of microns can be probed noninvasively, without any staining or mechanical slicing. As an important application of second harmonic imaging in ophthalmology, the modification of corneal ultrastructure using femtosecond laser intrastromal ablation is systematically investigated to evaluate next-generation refractive surgical approaches.
Meng Han, Leander Zickler, Günter Giese, Matthias Walter, Frieder H. Loesel, Josef F. Bille, "Second-harmonic imaging of cornea after intrastromal femtosecond laser ablation," Journal of Biomedical Optics 9(4), (1 July 2004). https://doi.org/10.1117/1.1756919

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