1 September 2004 Blood cell counting and classification by nonflowing laser light scattering method
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We present a nonflowing laser light scattering method for automatically counting and classifying blood cells. A linear charge-coupled device (CCD) and a silicon photoelectric cell (which is placed behind a pinhole plate on the CCD) form a double-detector structure: the CCD is used to detect the scattered light intensity distribution of the blood cells and the silicon photoelectric cell to complete the focusing process. An isotropic sphere, with relative refractivity near 1, is used to model the blood cell. Mie theory is used to describe the scattering of white blood cells and platelets, and anomalous diffraction, red blood cells. To obtain the size distribution of blood cells from their scattered light intensity distribution, the nonnegative constraint least-squares (NNLS) method combined with the Powell method and the precision punishment method are used. Both numerical simulation and experimental results are presented. This method can be used not only to measure the mean and the distribution of red blood cell size, but also to divide the white blood cells into three classes: lymphocytes, middle-sized cells, and neutrocytes. The experimental results show a linear relationship between the blood cell (both white and red blood cells) concentration and the scattered light intensity, and therefore, the number of blood cells in a unit volume can be determined from this relationship
Ye Yang, Zhenxi Zhang, Xinhui Yang, Joon Hock Yeo, Lijun Jiang, Dazong Jiang, "Blood cell counting and classification by nonflowing laser light scattering method," Journal of Biomedical Optics 9(5), (1 September 2004). https://doi.org/10.1117/1.1782572

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