1 September 2004 Parallel single molecule detection with a fully integrated single-photon 2×2 CMOS detector array
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Abstract
We present parallel single molecule detection (SMD) and fluorescence correlation spectroscopy (FCS) experiments with a fully integrated complementary metal oxide semiconductor (CMOS) single-photon 2×2 detector array. Multifocal excitation is achieved with a diffractive optical element (DOE). Special emphasis is placed on parallelization of the total system. The performance of the novel single-photon CMOS detector is investigated and compared to a state-of-the-art single-photon detecting module [having an actively quenched avalanche photodiode (APD)] by measurements on free diffusing molecules at different concentrations. Despite the order of magnitude lower detection efficiency of the CMOS detector compared to the state-of-the-art single-photon detecting module, we achieve single molecule sensitivity and reliably determine molecule concentrations. In addition, the CMOS detector performance for the determination of the fraction of slowly diffusing molecules in a primer solution (two-component analysis) is demonstrated. The potential of this new technique for high-throughput confocal-detection-based systems is discussed.
Michael Gösch, Alexandre Serov, Alexis Rochas, Hans Blom, Tiemo Anhut, Pierre-Andre Besse, Radivoje Popovic, Theo Lasser, Rudolf Rigler, "Parallel single molecule detection with a fully integrated single-photon 2×2 CMOS detector array," Journal of Biomedical Optics 9(5), (1 September 2004). https://doi.org/10.1117/1.1781668
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