3 September 2015 Local adaptive approach toward segmentation of microscopic images of activated sludge flocs
Author Affiliations +
J. of Electronic Imaging, 24(6), 061102 (2015). doi:10.1117/1.JEI.24.6.061102
Activated sludge process is a widely used method to treat domestic and industrial effluents. The conditions of activated sludge wastewater treatment plant (AS-WWTP) are related to the morphological properties of flocs (microbial aggregates) and filaments, and are required to be monitored for normal operation of the plant. Image processing and analysis is a potential time-efficient monitoring tool for AS-WWTPs. Local adaptive segmentation algorithms are proposed for bright-field microscopic images of activated sludge flocs. Two basic modules are suggested for Otsu thresholding-based local adaptive algorithms with irregular illumination compensation. The performance of the algorithms has been compared with state-of-the-art local adaptive algorithms of Sauvola, Bradley, Feng, and c-mean. The comparisons are done using a number of region- and nonregion-based metrics at different microscopic magnifications and quantification of flocs. The performance metrics show that the proposed algorithms performed better and, in some cases, were comparable to the state-of the-art algorithms. The performance metrics were also assessed subjectively for their suitability for segmentations of activated sludge images. The region-based metrics such as false negative ratio, sensitivity, and negative predictive value gave inconsistent results as compared to other segmentation assessment metrics.
© 2015 SPIE and IS&T
Muhammad Burhan Khan, Humaira Nisar, Choon Aun Ng, Po Kim Lo, Vooi Voon Yap, "Local adaptive approach toward segmentation of microscopic images of activated sludge flocs," Journal of Electronic Imaging 24(6), 061102 (3 September 2015). https://doi.org/10.1117/1.JEI.24.6.061102

Image segmentation

Image processing algorithms and systems

Image processing


Image analysis

Algorithm development

Detection and tracking algorithms


Aerial image: from straight lines to rectangles
Proceedings of SPIE (September 16 1994)
Spot identification on 2D electrophoresis gel images
Proceedings of SPIE (October 27 2006)
Image analysis of multiple moving wood pieces in real time
Proceedings of SPIE (February 15 2006)

Back to Top