22 August 2017 Estimation of subject coregistration errors during multimodal preclinical imaging using separate instruments: origins and avoidance of artifacts
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Abstract
We use high-resolution μCT data in multiple experiments to estimate the sources of error during coregistration of images acquired on separate preclinical instruments. In combination with experiments with phantoms, we completed in vivo imaging on mice, aimed at identifying the possible sources of registration errors, caused either by transport of the animal, movement of the animal itself, or methods of coregistration. The same imaging cell was used as a holder for phantoms and animals. For all procedures, rigid coregistration was carried out using a common landmark coregistration system, placed inside the imaging cell. We used the fiducial registration error and the target registration error to analyze the coregistration accuracy. We found that moving an imaging cell between two preclinical devices during a multimodal procedure gives an error of about 200  μm at most. Therefore, it could not be considered a source of coregistration errors. Errors linked to spontaneous movements of the animal increased with time, to nearly 1 mm at most, excepted for body parts that were properly restrained. This work highlights the importance of animal intrinsic movements during a multiacquisition procedure and demonstrates a simple method to identify and quantify the sources of error during coregistration.
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)
Jean-Philippe Dillenseger, Jean-Philippe Dillenseger, Christian Goetz, Christian Goetz, Amira Sayeh, Amira Sayeh, Chris Healy, Chris Healy, Isabelle Duluc, Isabelle Duluc, Jean-Noël Freund, Jean-Noël Freund, André Constantinesco, André Constantinesco, Gaëlle Aubertin-Kirch, Gaëlle Aubertin-Kirch, Philippe Choquet, Philippe Choquet, } "Estimation of subject coregistration errors during multimodal preclinical imaging using separate instruments: origins and avoidance of artifacts," Journal of Medical Imaging 4(3), 035503 (22 August 2017). https://doi.org/10.1117/1.JMI.4.3.035503 . Submission: Received: 27 April 2017; Accepted: 24 July 2017
Received: 27 April 2017; Accepted: 24 July 2017; Published: 22 August 2017
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