3 July 2014 Total internal reflection–based module for fluorescence and absorbance detection
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Abstract
We present a miniaturized polymer-based micro-optical detection unit for ultraviolet and visible laser-induced fluorescence (LIF) and absorbance (ABS) analysis with an interaction length of 3 mm. We use nonsequential optical ray tracing simulations to model the system and to optimize its performance with respect to optical efficiency and system complexity. The design features a compact optical system combining total internal reflection (TIR) mirrors and refractive optics. The detection module is prototyped with deep proton writing in 2-mm-thick polymethylmethacrylate and quantitatively characterized using a three-dimensional coordinate measurement machine. We demonstrate the proof-of-concept of this TIR mirror–based module for coumarin 480 obtaining limits of detection of 50 pM and 500 nM for LIF and ABS, respectively.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Tom Verschooten, Tom Verschooten, Heidi Ottevaere, Heidi Ottevaere, Michael Vervaeke, Michael Vervaeke, Jürgen Van Erps, Jürgen Van Erps, Hugo Thienpont, Hugo Thienpont, } "Total internal reflection–based module for fluorescence and absorbance detection," Journal of Micro/Nanolithography, MEMS, and MOEMS 13(3), 033001 (3 July 2014). https://doi.org/10.1117/1.JMM.13.3.033001 . Submission:
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