1 July 2009 Obstacles to the production of protein microarray cassettes
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Abstract
We examine the necessary technologies to be mastered in order to build a practical microarray-based immunoassay cassette and its processing station for protein analysis. The interdependence of surface chemistry, dye stability, and imaging are outlined, showing why a treated 100-nm film of nitrocellulose adhered by an intervening layer to glass offers an efficacious surface for immobilizing an array of protein probes. The properties of a storage surface to support in desiccated form, fluidize and transport additional reagents are outlined, and a practical solution proposed. Wet and dry imaging are compared. The steps and functions expected for an assay platform comprising a processing station and biochip cassette are identified. The performance of a successful benchtop automated multiplex immunoassay system is briefly described.
© (2009) Society of Photo-Optical Instrumentation Engineers (SPIE)
Jean I. Montagu, Herman DeWeerd, Nathan Tyburczy, Natalia Rodionova, Peter Maimonis, "Obstacles to the production of protein microarray cassettes," Journal of Micro/Nanolithography, MEMS, and MOEMS 8(3), 033013 (1 July 2009). https://doi.org/10.1117/1.3158071 . Submission:
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