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Three-dimensionally ordered photonic crystals were grown using self-assembly technique from Rhodamine-B dye doped polystyrene micro-spheres resulting in a stop band at 611 nm overlapping the emission spectrum of the dye. When excited at a wavelength away from the stop band, using a frequency-doubled Nd:YAG laser, the crystal showed angle-dependent suppression of spontaneous emission of the dye in the wavelength range of the photonic stop band and enhancement at the band edge, in reflection and transmission geometries. Spectral narrowing, a sharp threshold and a highly directional emission, all indicative of stimulated emission, were observed from the active photonic crystal matrix.