1 January 2010 Numerical simulation of near-field fluorescence correlation spectroscopy using a fiber probe
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Abstract
Numerical simulation of fluorescence correlation spectroscopy (FCS) based on near-field scanning optical microscopy (NSOM) was performed. A finite-difference time-domain method was employed to calculate the electric field distribution in the vicinity of the NSOM aperture and the Brownian motion of nanoparticles was reproduced by a Monte Carlo simulation. The physical validity of the simulation result was confirmed by comparing it with a theoretical calculation using a Gaussian observation volume, which is applied to FCS based on conventional optical microscopy.
Kazuo Kasahara, Toshiharu Saiki, "Numerical simulation of near-field fluorescence correlation spectroscopy using a fiber probe," Journal of Nanophotonics 4(1), 043502 (1 January 2010). https://doi.org/10.1117/1.3319569 . Submission:
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