10 May 2012 Effect of surface functionalization of porous silicon microcavities on biosensing performance
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Surface functionalization methods were investigated for their effects on the sensing performances of porous silicon (p-Si) microcavities when used for detection of biomolecules. These microcavities were fabricated to reveal reflectivity pass-band spectra in the visible and near-infrared spectral regime. In one approach, the devices were thermally oxidized and functionalized to ensure covalent binding of molecules. In the second approach, the as-etched p-Si surface was modified with adhesion peptides, isolated via phage display, that present high binding capacity for silicon. Functionalization and molecular binding events were monitored via reflectometric interference spectra as shifts in the resonance peaks of the cavity structure due to changes in the refractive index when a biomolecule is attached to the large internal surface of p-Si. Improved sensitivity was obtained owing to the peptide interface linkers between the p-Si and biological molecules compared to the silanized devices. Investigating the formation of peptide-Si interface layer via X-ray photoelectron spectroscopy, scanning tunneling microscopy, and scanning electron microscopy, we found that peptides form nanometer-thin layers on the Si surface and that their binding energy depends on the sequence of the peptide.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE)
Marta Martin, Marta Martin, Laurent Massif, Laurent Massif, Elias Estephan, Elias Estephan, Mari-Belle Saab, Mari-Belle Saab, Thierry Cloitre, Thierry Cloitre, Csilla Gergely, Csilla Gergely, Frédéric J. G. Cuisinier, Frédéric J. G. Cuisinier, Christian Larroque, Christian Larroque, Vivechana Agarwal, Vivechana Agarwal, Guy Le Lay, Guy Le Lay, } "Effect of surface functionalization of porous silicon microcavities on biosensing performance," Journal of Nanophotonics 6(1), 061506 (10 May 2012). https://doi.org/10.1117/1.JNP.6.061506 . Submission:

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