12 March 2015 Multimodal reconstruction of microvascular-flow distributions using combined two-photon microscopy and Doppler optical coherence tomography
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Abstract
Computing microvascular cerebral blood flow (μCBF) in real cortical angiograms is challenging. Here, we investigated whether the use of Doppler optical coherence tomography (DOCT) flow measurements in individual vessel segments can help in reconstructing μCBF across the entire vasculature of a truncated cortical angiogram. A μCBF computational framework integrating DOCT measurements is presented. Simulations performed on a synthetic angiogram showed that the addition of DOCT measurements, especially close to large inflowing or outflowing vessels, reduces the impact of pressure boundary conditions and estimated vessel resistances resulting in a more accurate reconstruction of μCBF. Our technique was then applied to reconstruct microvascular flow distributions in the mouse cortex down to 660  μm by combining two-photon laser scanning microscopy angiography with DOCT.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
Louis Gagnon, Louis Gagnon, Sava Sakadžić, Sava Sakadžić, Fréderic Lesage, Fréderic Lesage, Emiri T. Mandeville, Emiri T. Mandeville, Qianqian Fang, Qianqian Fang, Mohammad A. Yaseen, Mohammad A. Yaseen, David A. Boas, David A. Boas, } "Multimodal reconstruction of microvascular-flow distributions using combined two-photon microscopy and Doppler optical coherence tomography," Neurophotonics 2(1), 015008 (12 March 2015). https://doi.org/10.1117/1.NPh.2.1.015008 . Submission:
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