Voltage-sensitive dye imaging during functional development of the embryonic nervous system: a brief review with special thanks to Professor Larry Cohen

Yoko Momose-Sato; Katsushige Sato

doi:10.1117/1.NPh.2.2.021009

3 February 2015 Voltage-sensitive dye imaging during functional development of the embryonic nervous system: a brief review with special thanks to Professor Larry Cohen

Yoko Momose-Sato, Katsushige Sato

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Neurophotonics, Vol. 2, Issue 2, 021009 (February 2015). https://doi.org/10.1117/1.NPh.2.2.021009

Abstract

Investigating the developmental organization of the embryonic nervous system is one of the major challenges in the field of neuroscience. Despite their significance, functional studies on the vertebrate embryonic central nervous system (CNS) have been hampered by the technical limitations associated with conventional electrophysiological methods. The advent of optical techniques using voltage-sensitive dyes, which were developed by Dr. Cohen and his colleagues, has enabled electrical activity in living cells to be monitored noninvasively and also facilitated the simultaneous recording of neural responses from multiple regions. Using optical recording techniques, it is now possible to follow the functional organization of the embryonic CNS and image the spatiotemporal dynamics involved in the formation of this neural network. We herein briefly reviewed optical studies on the embryonic CNS with a special emphasis on methodological considerations and the study of neuronal circuit formation, which demonstrates the utility of fast voltage-sensitive dye imaging as a powerful tool for elucidating the functional organization of the embryonic CNS.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Yoko Momose-Sato and Katsushige Sato "Voltage-sensitive dye imaging during functional development of the embryonic nervous system: a brief review with special thanks to Professor Larry Cohen," Neurophotonics 2(2), 021009 (3 February 2015). https://doi.org/10.1117/1.NPh.2.2.021009

Published: 3 February 2015

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KEYWORDS

Nervous system

Voltage sensitive dyes

Imaging systems

Neurons

Nerve

Optical recording

Tissues

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