23 July 2015 All-optical bidirectional neural interfacing using hybrid multiphoton holographic optogenetic stimulation
Author Affiliations +
Neurophotonics, 2(3), 031208 (2015). doi:10.1117/1.NPh.2.3.031208
Our understanding of neural information processing could potentially be advanced by combining flexible three-dimensional (3-D) neuroimaging and stimulation. Recent developments in optogenetics suggest that neurophotonic approaches are in principle highly suited for noncontact stimulation of network activity patterns. In particular, two-photon holographic optical neural stimulation (2P-HONS) has emerged as a leading approach for multisite 3-D excitation, and combining it with temporal focusing (TF) further enables axially confined yet spatially extended light patterns. Here, we study key steps toward bidirectional cell-targeted 3-D interfacing by introducing and testing a hybrid new 2P-TF-HONS stimulation path for accurate parallel optogenetic excitation into a recently developed hybrid multiphoton 3-D imaging system. The system is shown to allow targeted all-optical probing of in vitro; cortical networks expressing channelrhodopsin-2 using a regeneratively amplified femtosecond laser source tuned to 905 nm. These developments further advance a prospective new tool for studying and achieving distributed control over 3-D neuronal circuits both in vitro and in vivo.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
Shir Paluch-Siegler, Hod Dana, Tom Mayblum, Inbar Brosh, Inna Gefen, Shy Shoham, "All-optical bidirectional neural interfacing using hybrid multiphoton holographic optogenetic stimulation," Neurophotonics 2(3), 031208 (23 July 2015). https://doi.org/10.1117/1.NPh.2.3.031208

Back to Top